Copper-Binding Compounds from Methylosinus trichosporium OB3b
نویسندگان
چکیده
منابع مشابه
Analysis of methane biodegradation by Methylosinus trichosporium OB3b
The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monito...
متن کاملIsolation of copper biochelates from Methylosinus trichosporium OB3b and soluble methane monooxygenase mutants.
Methylosinus trichosporium OB3b produces an extracellular copper-binding ligand (CBL) with high affinity for copper. Wild-type cells and mutants that express soluble methane monooxygenase (sMMO) in the presence and absence of copper (sMMOc) were used to obtain cell exudates that were separated and analyzed by size exclusion high-performance liquid chromatography. A single chromatographic peak, ...
متن کاملDetoxification of mercury by methanobactin from Methylosinus trichosporium OB3b.
Many methanotrophs have been shown to synthesize methanobactin, a novel biogenic copper-chelating agent or chalkophore. Methanobactin binds copper via two heterocyclic rings with associated enethiol groups. The structure of methanobactin suggests that it can bind other metals, including mercury. Here we report that methanobactin from Methylosinus trichosporium OB3b does indeed bind mercury when...
متن کاملGenome sequence of the obligate methanotroph Methylosinus trichosporium strain OB3b.
Methylosinus trichosporium OB3b (for "oddball" strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and th...
متن کاملEffect of copper speciation on whole-cell soluble methane monooxygenase activity in Methylosinus trichosporium OB3b.
Soluble methane monooxygenase (sMMO) activity in Methylosinus trichosporium OB3b was found to be more strongly affected as copper-to-biomass ratios changed in a newly developed medium, M2M, which uses pyrophosphate for metal chelation, than in nitrate mineral salts (NMS), which uses EDTA. When M2M medium was amended with EDTA, sMMO activity was similar to that in NMS medium, indicating that EDT...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1998
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.180.14.3606-3613.1998